P-15: Study Of the Effect of Nicotine - Melatoninon Sperm Parameters in Adult Mouse

Background: Cigarette smoking is associated with impairment of testicular function. Nicotine is a major component of cigarette smoke.In the present study we aimed to study the effect of administration of nicotine-melatonin on sperm parameters in adult mouse. Materials and Methods: Male NMRI mice were divided into 4 groups: 1) control(normal saline) group 2) nicotine treated group 3) melatonin treated group 4) Nicotinemelatonin treated group. Nicotine were administered in dose of 0.04mg/100g.bw(equivalent to heavy smokers) intraperitoneally for 14 days. Melatonin were administered in dose of 10 mg/kg for 5 days intraperitoneally. On 15 days after the treatment sperm samples were obtained from cauda epididymis. Parameters measured were sperm count, abnormal head and tail morphology and sperm motility. Motility were scored as fast progressive, slow progressive, shaking and immotile sperms. Statistical analysis was performed by ANOVA test Results: Nicotine caused a decrease in sperm count (p